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Winyoo Chowanadisai, Ph.D.

Chowanadisai

Nutrient metabolism, Gene mutations/polymorphisms, Brain development and function

Degree: PhD, Nutrition
Broad Expertise/Interests:
  • Cellular and animal models of neurodevelopment and brain disease
  • Interactions of nutrients and gene mutations/polymorphisms on brain health and function
Equipment/Techniques:
  • Primary mouse/rat neuron and neuronal cell-line cultures
  • Other cell lines (HEK293, CHO) for heterologous gene expression
  • Loss of function approaches: shRNA, CRISPR
  • Gain of function approaches: gene cloning (PCR), non-viral plasmid transfection, epitope tagging
  • Promoter reporter assays: promoter cloning, luciferase assays
  • Xenopus embryo models for development (ISH, loss of function by morpholinos, can be adapted to CRISPR)
  • Knockout and transgenic (such as AD) mouse models
  • Various cellular techniques:
    • Cell culture, brightfield morphological analyses (like neurite outgrowth)
    • Fluorescence microscopy (live and fixed)
    • Live cell imaging using indicator fluorescence dyes
    • Fluorescent proteins (like GFP)
    • Currently developing FRET-sensor protocols
    • Laser confocal microscopy
    • Protein-protein interactions (FRET, BiFC)
    • Image analyses with NIH ImageJ/Fiji
  • Various methods of gene expression: QRTPCR, currently developing RNA-seq protocol, other aspects of RNA analysis (deep PCR amplicon sequencing, splice analysis, non-coding RNAs, etc.)
  • Various protein biochemical assays: (Western blotting, ELISA, enzymatic assays)
  • Basic bioinformatic/computational approaches:
    • BLAST (including non-conventional genomes)
    • Comparative genome analyses
    • Predicted translation
    • Domain predictions
    • Promoter sequence analyses
    • Genomic DNA isolation and sequence analyses
  • Human genetic samples
    • Collection and isolation via saliva and blood for genomic DNA
    • Polymorphism and mutation detection
Seeking Collaborators with Skill Sets:
  • Rodent model behavioral phenotyping (i.e. Morris water maze)
  • Rodent model brain histology (Golgi stain, Nissl stain, IHC, etc.)
  • Primary mouse neurons and lentiviral transduction
  • Non-mammalian, aquatic animal models
  • Clinical samples (with patient history) of neurodevelopmental disorders
Research Interests: My research interests involve using genomic datasets to uncover novel genes necessary for brain development and function.
Funding in the Last Three Years:

OCAST, USDA/OAES, USDA/NIFA, CIRCA Pilot

Publication Highlights:

Chowanadisai W*, Graham DM, Keen CL, Rucker RB, Messerli MA. Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). PNAS. 2013; 110: 9903-9908. *corresponding author

Chowanadisai W*, Graham DM, Keen CL, Rucker RB, Messerli MA. A zinc transporter gene required for development of the nervous system. Communicative & Integrative Biology. 2013; 6: e26207. *corresponding author

Chowanadisai W*. Comparative genomic analysis of slc39a12/ZIP12: Insight into a zinc transporter required for vertebrate nervous system development. PLoS One. 2014; 9: e111535. *corresponding author

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